VALIDATED METHOD FOR ESTIMATION OF TOTAL FLAVONOIDS IN ROMANIAN PROPOLIS
Abstract
In this article there are two methods presented for determination of 2 groups of flavonoids: flavone/flavonols (FF) - AlCl3, and flavanones/dihydroflavonols (FD) - 2,4-dinitrophenylhidrazine. Together they sum up the total flavonoids (FvT) in propolis. Romanian propolis taken into analysis had an amount of 4% flavone/flavonols, 6% flavanones/dihydroflavonols and about 10% total flavonoids typical for propolis derived from Populus sp. Statistical correlation between data (FF, FD, FvT) obtained by sonication and by maceration present no significance difference between results.a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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