DETERMINATION OF FATTY ACIDS WITHIN BUBALINE MILK BY USE OF THE GAS-CROMATOGRAPHIC METHOD

Abstract

Investigations were conducted on a lot of bubalines raised within a household system. The ratio between saturated and unsaturated fatty acids encountered in the bubaline milk is rather constant. It can thus be asserted that apprehending the fatty acid concentration in bubaline milk and dairy products may confirm the authenticity and freshness of the dairy products in question. The analysis on fatty acids consisted in their extraction and processing through transesterification and gas-chromatographic (GS-MS) analysis. Therefore, the following stages were undergone: the extraction of total lipids, the obtainment of methylic esters in fatty acids, the separation and identification of methylic esters in fatty acids through gaseous- phase chromatography (GS). By means of the gas-chromatograph, we were able to identify the fatty acids with their corresponding standards, namely: capric acid, lauric acid, myristic acid, palmitic acid, palmitoleic acid, heptadecanoic acid, stearic acid, oleic acid, linoleic acid and arachidonic acid.