DNA FINGERPRINTING OF TULIPS USING AFLP ANALYSIS

Ioana Olivia BONDREA, Doru PAMFIL, Sjaak van HEUSDEN, Jaap van TUYL, Fien MEIJER-DEKENS, Mircea BONDREA, Radu SESTRAS, Meda LUCACI

Abstract


Tulip is one of the most important flower bulbs of spring days, mostly created by Dutch breeders. More than six thousands Tulip varieties are cultivated worldwide. The genome of tulip is 5000x bigger as the genome of arabidopsis (120.000 Kb) and consists of 12 chromosomes. Little information about genes and their position on the chromosomes is available. Botanical tulips are on their way to disappearing and almost unknown to the public. Tulip are also victims of urbanization which nibbles away constantly at de natural spaces (Tulipa aximensis disappeared from the wild in 1974). Botanical plants are adapting themselves to a particular and natural environment over a long period of time, more stable and furthermore resistant, contributing to natural balance. In connection with the France Tulipes Sauvages Association, which contributes to the protection of botanical tulips of France and whole world (4), we start a molecular study to explore the genetic variability of this species and to help the conservation of rare and endangered species, including the Romanian one. The AFLP method was performed as described by Vos et al. (1995) with some minor modifications. The study offered reproducible AFLP patterns, clear and labor saving DNA fingerprints of T. fosteriana 148 Cantate x Princeps, T. tubergeniana 97419, T. hungarica 97423, T. albertii 97274-3, T. turkestanica 97297-1, T. aitchisonii 20425, T. tarda 73125-4. We used en efficient enzyme primer combination M52G/E45. The study is extended also to other Tulipa species.

Keywords


AFLP genetic marker; Tulipa; DNA fingerprinting; PCR; selective nucleotides

Full Text:

PDF


DOI: http://dx.doi.org/10.15835/buasvmcn-asb:64:1-2:2308




University of Agricultural Sciences and Veterinary Medicine
3-5 Manastur St., 400372 Cluj-Napoca
Romania
Tel: +40-264-596.384 | Fax: +40-264-593.792 
biroupublicatii@usamvcluj.ro