Results on the Composition of Milk Fatty Acid in Milk of Buffaloes Compared with the Cow's Milk

  • VIOARA MIRESAN University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotehnologies, 3-5 Manastur Street, 400372 Cluj-Napoca
  • AURELIA PECE University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotehnologies, 3-5 Manastur Street, 400372 Cluj-Napoca
  • CAMELIA RADUCU University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotehnologies, 3-5 Manastur Street, 400372 Cluj-Napoca
Keywords: fatty acids, milk, buffaloes, cows

Abstract

The fatty acids analysis consisted in their extraction and processing for their analysis through transesterification and gas-chromatographic analysis GC. Therefore, the following stages were covered: total fat extraction, the obtaining of fatty acids methyl acids, the separation and identification of methyl esters through gaseous phase chromatography (GC). Fatty acids were identified by means of the gas-chromatograph, respectively those which had standards: the capric, lauric, mystiric, palmitic, palmitoleic acids, the heptadecanois, stearic, oleic, linoleic and arahidonic acids. As specialty literature data referring to the optimum derivatising method for buffalo milk fatty acids are contradictory, several methods have been tested and compared. The gas-chromatographic separation of components in a mixture depend on: the nature of the distribution isotherm; sample size; the nature of the vector gas; temperature programming and the eluant flow; phase nature; detector type.

Author Biographies

VIOARA MIRESAN, University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotehnologies, 3-5 Manastur Street, 400372 Cluj-Napoca
ANIMAL SCIENCE
AURELIA PECE, University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotehnologies, 3-5 Manastur Street, 400372 Cluj-Napoca
ANIMAL SCIENCE
CAMELIA RADUCU, University of Agricultural Sciences and Veterinary Medicine, Faculty of Animal Science and Biotehnologies, 3-5 Manastur Street, 400372 Cluj-Napoca
ANIMAL SCIENCE