Results on the Composition of Milk Fatty Acid in Milk of Buffaloes Compared with the Cow's Milk
Abstract
The fatty acids analysis consisted in their extraction and processing for their analysis through transesterification and gas-chromatographic analysis GC. Therefore, the following stages were covered: total fat extraction, the obtaining of fatty acids methyl acids, the separation and identification of methyl esters through gaseous phase chromatography (GC). Fatty acids were identified by means of the gas-chromatograph, respectively those which had standards: the capric, lauric, mystiric, palmitic, palmitoleic acids, the heptadecanois, stearic, oleic, linoleic and arahidonic acids. As specialty literature data referring to the optimum derivatising method for buffalo milk fatty acids are contradictory, several methods have been tested and compared. The gas-chromatographic separation of components in a mixture depend on: the nature of the distribution isotherm; sample size; the nature of the vector gas; temperature programming and the eluant flow; phase nature; detector type.a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
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