Determination of Six Sulfonamide Residues in Honey by HPLC with Fluorescence Detection

VICTORITA BONTA, LIVIU-ALEXANDRU MARGHITAS, DANIEL DEZMIREAN, OTILIA BOBIS

Abstract


Six sulfonamides (sulfanilamide, sulfacetamide, sulfadiazine, sulfathiazole, sulfamethazine, sulfamethoxazole) were separated and quantified in honey of different botanic origin using an HPLC-fluorescence detection method. After the acidic hydrolysis, sulfonamides were extracted from honey with a mixture of acetonitrile and dichloromethane, further clean-up on a C18 SPE cartridge and pre-column derivatization with fluorescamine. LC separation was performed on a RP C8 column in gradient mode, in 30 min. The limit of detection was determined at 2µg/kg for sulfadiazine and sulfamethazine, 3µg/kg for sulfanilamide, sulfacetamide and sulfathiazole and 5µg/kg for sulfamethoxazole. The sample clean-up was efficient and recoveries of the analytes in spiked honeys ranged from 47 to 79%.

Keywords


liquid chromatography; fluorescence; sulfonamides; honey

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:66:1-2:3360




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