Using Cellulases and Hemicellulases to Improve Better Extractionof Carotenoids from the Sepals of Physalis Alkekengi L.

Abstract

The aim of this study was to extract the carotenoids from the sepals of Physalis Alkekengi L. and to use cellulases and hemicellulases from the beginning of the extraction for better extraction. The concentration of the enzymes used was different also the time of hydrolysis. Total carotenoid content was measured using the spectrophotometric method. HPLC-DAD was used to determine the carotenoid profile for the extracts. HPLC Analysis of the extract indicates that the original carotenoid profile is not altered. The enhanced extraction system appears to be very competitive when compared to the classical extraction using solvents