Using Cellulases and Pectinases to Improve Better Extraction of Carotenoids from the Marigold Flowers (Tagetes erecta L.)

Andreea BUNEA, Augusta LUJERDEAN, Adela PINTEA, Sanda ANDREI, Claudiu BUNEA, Constantin BELE


The aim of this study was to extract the carotenoids from the marigold flowers and to use cellulases, hemicellulases and pectinases from the beginning of the extraction for better extraction. The concentration of the enzymes used was different also the time of hydrolysis. Total carotenoid content was measured using the spectrophotometric method. HPLC-DAD was used to determine the carotenoid profile for the extracts. HPLC analysis of the extract indicates that the original carotenoid profile is not altered. The enhanced extraction system appears to be very competitive when compared to the classical extraction using solvents.


marigold flowers, carotenoids, cellulases, pectinases, yield

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