Development of Methods for DNA Extraction from Leaves and Must Grapes

Abstract

The wine sector is one of the economically most important agricultural activities in the world. An enormous diversity of Vitis vinifera L. varieties (cultivars) can be used in the production of wine, although only a small number is of commercial importance.
Ten grapevine varieties have been analysed. Five varieties are used for red wine production and five for white wine production. Two types of samples were analyzed: young leaves and must. Leaves were collected at the S.D.E Cluj. Must was obtained right after grape crushing, containing the skin and the solid parts of the flesh.
Three different methods were tested: Lodhi et al., (1994) modified by Pop et al., (2003); Faria et al., (2000); Sambrook et al., (1989). The first method was used for DNA extraction from leaves and also from must. Analyses with DNA extracted from leaves and monovarietal musts were performed using the following microsatellite loci: VVS2, VVS5, VVMD5, VVMD7, ssVrZAG 47, ssVrZAG 62 and ssVrZAG79.