Acaricides Determination from Honey through GC-MS Technique Using Different Extraction Methods

Liviu Al. MARGHITAS, Victorita BONTA, Cristina M. MIHAI, Daniel S. DEZMIREAN

Abstract


Honey was subjected to GC-MS analysis in order to identify and quantify acaricides residues (2,4-dimethylaniline, brompropilate, amitraz, coumaphos, fluvalinate). Two rapid methods were compared for the extraction and purification of acaricides from honey. First method uses as extraction solvent acetonitrile in the presence of high quantities of salts, which underwent a clean up step known like dispersive solid-phase extraction dSPE PSA (QuEChERS method). In the second method the acaricides were eluted with dichloromethane from the mixture of the sample with diatomaceous earth. Both methods offered the extraction of all mentioned acaricides, but the second one offered higher recoveries, lower costs and was less time consuming for sample preparation. LOD for all acaricides ranged from 0.8-8.3 μg/kg and for LOQ between 1 and 9.7 μg/kg. Higher recovery rates (90 - 108%) were obtained when sample preparation involved diatomaceous earth. 


Keywords


honey, gas chromatography, mass spectrometry, acaricides

Full Text:

PDF


DOI: http://dx.doi.org/10.15835/buasvmcn-asb:69:1-2:8382


University of Agricultural Sciences and Veterinary Medicine
3-5 Manastur St., 400372 Cluj-Napoca
Romania
Tel: +40-264-596.384 | Fax: +40-264-593.792 
biroupublicatii@usamvcluj.ro