In Vitro Experimental Researches Regarding the Treatment with Phytoregulators of Orach (Atriplex hortensis L.)

Oana Livadariu

Abstract


Orach (Atriplex hortensis L.) is a halophyte plant, cultivated ever since the ancient times, used in the food industry, biotherapy, human medicine and apiculture. But, then it has a germinal capacity of about 25% and a duration of two years. These characteristics make the A. hortensis L. a plant with economic potential exploitable via biotechnological methods. These methods use phytoregulators as biochemical instruments capable of modeling somatic type explants cultivated in vitro for the purposes of the induction, generation and materialization of the properties characteristic of stem type cells. These properties are especially relevant by the use of auxinic or cytokininic type phytoregulators.

The experimental researches described in this study has aimed at testing treatments with exogenous auxins or exogenous cytokinins of A. hortensis L., on somatic type explants cultivated in vitro, for the purposes of the induction, generation and materialization of properties characteristic of stem type cells, transformed in the form of callogenesis, caulogenesis and rhizogenesis.

The vegetal biological material used for the testing of treatments with exogenous auxins (IAA or 2,4,5-T) or exogenous cytokinins (TDZ or 2-iP) has consisted in two varieties of orach (A. hortensis L. var. rubra and A. hortensis L. var. viridis) and five types of somatic explants (leaf, cotyledon, epicotyl, hypototyl and radiclet). The research methods have been concordance with the pursued aim, and accord with the plant biotechnologies.

Out of the treatments with exogenous auxins, the best result has been obtained using the 2,4,5-T 0.50mg/l exogenous auxin on A. hortensis L. var. viridis, on radicle type explants, since it has allowed the induction, generation and materialization of the callogenesis and indirect rhizogenesis processes. At the same time, out of the treatments with exogenous cytokinins, the treatment with TDZ 0.75mg/l exogenous cytokinins has generated the best result on A. hortensis L. var. rubra, on cotyledon type explants, since it has allowed the induction, generation and materialization of the indirect callogenesis and caulogenesis processes.

The results of the experimental researches described in this study demonstrate that the application of treatments with exogenous auxins or exogenous cytokinins on orach (A. hortensis L.), on somatic type explants cultivated in vitro, allows the induction, generation and materialization of properties characteristic of stem type cells, transformed in the form of callogenesis, indirect caulogenesis and indirect rhizogenesis.

Keywords


orach, Atriplex hortensis L., exogenous auxins, exogenous cytokinins

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:70:2:9139




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