Determination of trans-10-hydroxy-2-decenoic acid (10-HDA) in Transylvanian Royal Jelly

Liviu Alexandru Marghitas, Lavinia Ioana Tomos, Daniel Severus Dezmirean, Otilia Bobis, Victorita Bonta, Rodica Margaoan, Bogdan Gherman


This paper contains a summary about determination 10-HDA of fresh Royal Jelly. The 10-HDA content in Transylvania samples of RJ was analyzed using an HPLC-PDA method. The chromatographic condition were: isocratic system, reverse phase C18 column, autosampler, diode array UV-VIS detector adjusted to 210 nm, mobile phase composed by methanol/water (60:40) at pH=2.5 adjusted with phosphoric acid.The 10-HDA content of the samples analyzed seem to have two ranges 0.75-3.8%. This is the first data regarding 10-HDA content of Romanian RJ.


Royal Jelly, 10-hydroxy-2-decenoic acid, HPLC analysis

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