EFFECT OF LEPTIN ON OVOGENESIS DURING DEVELOPMENT IN FEMALE WISTAR RAT

Ghouri Imane Institute of Veterinary Sciences, University of Blida -1, Route de Soumâa, BP 270, 09 000, Blida; Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Hanane Bouchefa Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Amina Boubekri Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Amel Ghozlani Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Mohamed Bachir Pacha Institute of Veterinary Sciences, University of Blida -1, Route de Soumâa, BP 270, 09 000, Blida
Rachid Kaidi Institute of Veterinary Sciences, University of Blida -1, Route de Soumâa, BP 270, 09 000, Blida
Fatima Hadj-Bekkouch Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers

DOI: https://doi.org/10.15835/agrisp.v107i3-4.13145

Keywords: Leptin, ovarian weight, enumeration, follicle, atresia

Abstract

The present study aims to investigate the effect of leptin treatment on ovarian weight and ovarian follicle enumeration during development in female Wistar rats. The experiment is carried out on 30 and 60-day-old females. Leptin diluted in 9‰ NaCl is injected daily for 5 days intraperitoneally at a dose of 8 μg / 100 g of body weight to the experimental females. The same volume of the NaCl solution is injected into the controls. Animals are sacrificed 24 hours after the last injection. Ovaries are removed and weighed. The right ovaries are fixed in 10% formaldehyde for histological study. The results reveal a non-significant increase in absolute and relative mean weights of the right and left ovaries in leptin-treated groups compared to controls in both immature and adult rats. The histological study reveals that in 30-day-old females, the number of atretic follicles is most important compared to normal follicles after leptin-treatment, which suggests that leptin appears to stimulate cellular apoptosis at immature age. These results allow us to conclude that leptin seems to influence ovarian weight and follicular atresia.

Published

2018-12-15

Issue

Vol. 107 No. 3-4 (2018): AGRICULTURA

Section

Research articles

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

The papers published in the journal are licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

Open Access Journal: The journal allow the author(s) to retain publishing rights without restrictions. Authors are allowed to read, download, copy, distribute, print, search, or link to the full texts of the articles, or use them for any other lawful purpose, without asking prior permission from the publisher or the author.

Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under the Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).