EFFECT OF LEPTIN ON FOLLICULOGENESIS DURING DEVELOPMENT IN FEMALE WISTAR RAT

Imane Ghouri Laboratory of Biotechnologies related to Animal Reproduction, Institute of Veterinary Sciences, University of Blida-1, Route de Soumâa, BP 270, 09 000, Blida Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Hanane Bouchefa Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Amina Boubekri Laboratory of Research on Arid Lands (LRZA), Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Amel Ghozlani Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers
Mohamed Bachir Pacha Laboratory of Biotechnologies related to Animal Reproduction, Institute of Veterinary Sciences, University of Blida-1, Route de Soumâa, BP 270, 09 000, Blida
Rachid Kaidi Laboratory of Biotechnologies related to Animal Reproduction, Institute of Veterinary Sciences, University of Blida-1, Route de Soumâa, BP 270, 09 000, Blida
Fatima Hadj-Bekkouche Laboratory of Endocrinology, Faculty of Biological Sciences, University of Sciences and Technology Houari Boumediene (USTHB), BP 32, El Alia, Bab Ezzouar, 16 111, Algiers

DOI: https://doi.org/10.15835/agrisp.v115i3-4.13908

Keywords: Leptin, Body weight, Folliculogenesis, Gonadotropins, Progesterone, Rat

Abstract

This study aims to investigate the effect of leptin on ovaries in female Wistar rat during development. Rats were divided into two groups: 30 and 60-day-old females. Experimental treated females were administrated 8 μg/100 g body weight intraperitoneal leptin injections daily for 5 days, while controls received the same volume of saline solution. Leptin treatment resulted in a significant decrease in body weight (150.50 ± 4.02 vs 157.78 ± 3.51 g, p < 0.05*) and a very significant decrease in food intake (14.75 ± 0.31 vs 16.33 ± 1.22 g, p < 0.01**) in the 60-day group. Vaginal smears did not reveal an estrus stage after treatment. Histological examination revealed a suspension of ovulation and an acceleration of the luteinization of granulosa cells in adult treated group in relation to the elevation of LH (0.301 ± 0.032 vs 0.189 ± 0.020 IU/L, p < 0.05*) and progesterone (41.60 ± 4.58 vs 33.39 ± 5.13 ng/mL, p > 0.05). For FSH, the difference between controls and treated females was not significant in both ages (p > 0.05). Leptin appears to be involved in the regulation of body weight, folliculogenesis, the ovulatory process and luteogenesis by control of gonadotropic hormones and progesterone at the age of 60 days but not at 30 days, when leptin receptors are probably not expressed yet.

Published

2021-01-18

Issue

Vol. 115 No. 3-4 (2020): AGRICULTURA

Section

Research articles

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