INTEREST OF PROGESTERONE, BETAHYDROXY-BUTYRATE, UNESTERIFIED FATTY ACIDS, CORTISOL AND C-REACTIVE PROTEIN IN PREDICTING THE RESULTS OF ARTIFICIAL INSEMINATION IN DAIRY COW

KALEM Ammar, BOUABBA Sadia, YAHIA Achour, ABDELLI Amine, METNA Boussad, KAIDI Rachid

Abstract


The objective of our work is the study of certain risk factors for infertility in dairy cows. It was carried out in the Tizi Ouzou region for the period from January 2020 to September 2020. This study is interested in establishing the link between repeated failures of artificial insemination and certain blood metabolites. Blood samples were taken shortly before artificial insemination for the determination of progesterone and certain metabolites: Glucose, cholesterol, beta-hydroxy-butyrate (BHB), non-esterified fatty acids (AGNE), urea, albumin, cortisol, aspartate aminotransferase (AST) and C-Reactive Protein (CRP). The descriptive analysis of the data revealed high frequencies of cows inseminated in luteal phase (28.89%), in negative energy balance (17.78%) and of stressed cows (46.67%), which had levels in elevated cortisol (22.96ng / ml). The concentrations of the metabolites in pregnant cows were within the limits of the reference values; on the other hand we recorded an increase in the non-pregnant ones.

The relational study by principal component analysis (PCA), showed that the increase in the concentrations of some metabolites and the decrease of some others, were associated with a failure of artificial insemination. The correlation matrix shows positive correlations on the one hand, very highly significant between BHB and AGNE (r = 0.70), as well as between blood sugar and cortisol (r = 0.58); on the other hand highly significant between BHB and AST (r = 0.38). Significant positive correlations were observed between BHB contents and cholesterolemia (r = 0.30), cholesterolemia and cortisolemia (r = 0.29), AST and CRP (r = 0.36), AST and albuminemia( r = 0.36), AST and P4 (r = 0.36) and finally between P4 and albuminemia (r = 0.31). In addition, there is a highly significant negative correlation between the success of artificial insemination (gestation) and the levels of progesterone (r = - 0.33), cortisol (r = 0.38) and BHB (r = 0, 39). These results confirm that infertility is characterized by the multiplicity of factors involved.


Keywords


cow, fertility, energy balance, artificial insemination, heat, metabolites



DOI: http://dx.doi.org/10.15835/agrisp.v120i3-4.14282



Copyright (c) 2022 KALEM Ammar, BOUABBA Sadia, YAHIA Achour, ABDELLI Amine, METNA Boussad, KAIDI Rachid

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