PROPAGATION BY TISSUE CULTURE OF SOME HOSTA TAXA FROM THE COLLECTION OF THE "ALEXANDRU CIUBOTARU" NATIONAL BOTANICAL GARDEN (INSTITUTE)

  • Aliona Glijin
  • Ion Roșca
  • Tatiana Sîrbu
  • Nina Ciorchină
  • Irina Sfeclă
  • Maria Tabără
Keywords: Hosta, 'Blue Mouse Ears', 'Lemon Lime', micropropagation, in vitro, ex vitro, growth medium, explant, microcloning, rhizogenesis, acclimatization

Abstract

The goal of our research consisted in the development of a micropropagation technology for hostas, providing high-quality planting material in a short time and determining the optimal adaptation and acclimatization conditions for Hosta plants obtained by tissue culture. Two cultivars of Hosta from the collection of the "Alexandru Ciubotaru" National Botanical Garden (Institute) have been studied: Hosta 'Blue Mouse Ears' and Hosta 'Lemon Lime'. Underground buds were used as explants. Two sterilizing agents were applied in the aseptic technique: mercury chloride (0.1%) and sodium hypochlorite (ACE, 10%). The survival rate six weeks after inoculation was almost 70% in the taxon 'Blue Mouse Ears' and 94% in the taxon 'Lemon Lime' sterilized with mercury chloride and over 73% in the taxon 'Blue Mouse Ears' and of about 65% in the taxon 'Lemon Lime' sterilized with sodium hypochlorite solution, thus both methods of sterilization are considered effective for the asepsis of Hosta explants. The supplementation of the MS growth medium with BAP, at a concentration of 0.5 mg/L, in combination with 0.5 mg/L NAA led to favourable results in explants of both taxa, inducing the appearance of microclones as early as the fifth week from inoculation. The percentage of in vitro rooting on the rhizogenesis medium ((MS (50%), IAA (1.0 mg/L), sucrose (30.0 g/L), agar (7.0 g/L)) was about 90%. The acclimatization percentage was very high, constituting 80-90% at the first stage (in the climate-controlled room), and the survival percentage at the second stage (in the greenhouse) – 100%. The technology adapted in the Biotechnology and Embryology Laboratory can be successfully used in the multiplication of representatives of the genus Hosta by tissue culture.

Published
2023-07-12
Section
Research articles