Dissociation of Rhodococcus rhodochrous Population after the Whole Cells Immobilization

  • Inna RASTIMESINA Institute of Microbiology and Biotechnology, MECR, Academiei 1, Chisinau, MD-2028
  • Olga POSTOLACHI Institute of Microbiology and Biotechnology, MECR, Academiei 1, Chisinau, MD-2028
  • Valentina JOSAN Institute of Microbiology and Biotechnology, MECR, Academiei 1, Chisinau, MD-2028
Keywords: immobilization, organic/inorganic matrix, dissociation.

Abstract

Six agricultural organic wastes and three inorganic matrices were selected for rhodococci whole cells immobilization. The degree of immobilization of rhodococci cells varied from 6.20% to 34.30% on organic matrices. A high level of Rhodococcus rhodochrous CNMN-Ac-05 cells immobilization was demonstrated on inorganic matrices, it was from 69.25% to 97.30%. After the contact with support the strain dissociated, forming, in addition to original S type, rough (R) and altercolour smooth (S) types. Immobilization of rhodococci cells on organic supports led to the appearance of phenotypic heterogeneity from 0.34% to 3.26%. On inorganic matrices the variability of rhodococci was 0.88-1.05%.

Published
2021-05-14