New Method of Obtaining Proteolytic Enzymatic Preparation from Fungal Strain Fusarium gibbosum CNMN FD 12

  • Steliana CLAPCO Institute of Microbiology and Biotechnology, Technical University of Moldova, Academiei str. 1, Chisinau, MD2028, Republic of Moldova
  • Alexandra CILOCI Institute of Microbiology and Biotechnology, Technical University of Moldova, Academiei str. 1, Chisinau, MD2028, Republic of Moldova
  • Elena DVORNINA Institute of Microbiology and Biotechnology, Technical University of Moldova, Academiei str. 1, Chisinau, MD2028, Republic of Moldova
  • Svetlana LABLIUC Institute of Microbiology and Biotechnology, Technical University of Moldova, Academiei str. 1, Chisinau, MD2028, Republic of Moldova
DOI: https://doi.org/10.15835/buasvmcn-fst:2023.0019
Keywords: coordination compound, fungi, lab-scale fermenter, proteases

Abstract

Proteases, that are able to hydrolyze the peptide bonds in proteins and polypeptides, occupy a key position in diverse fields, such as food, agriculture, animal feed. Considering the increasing demand for microbial proteases, identification of new way for improving the productivity of producers is of great importance. The aim of the present study was to evaluate the effect of a new coordination compound [Fe(H2L)(H2O)2](NO3)3∙2.5H2O  (where H2L = 2,6-diacetylpyridine bis(picolinoylhydrazone)) on the activity of proteolytic complex synthesized by the micromycete Fusarium gibbosum CNMN FD 12 under the submerged fermentations in shake flask, as well as in lab-scale fermenter. It was found that [Fe(H2L)(H2O)2](NO3)3∙2.5H2O exerts a significant positive effect on proteolytic activity in F. gibbosum CNMN FD 12 both in flasks and bioreactor and can be used as stimulator and regulator of proteases activity. By varying the concentration of the coordination compound and the duration of cultivation, it is possible to obtain proteolytic complexes enriched in different types of proteases (acid, neutral or alkaline), depending on the practical needs.

Published
2024-05-15
Issue
Vol. 81 No. 1 (2024): BULLETIN OF UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE CLUJ-NAPOCA. FOOD SCIENCE AND TECHNOLOGY
Section
Research Articles
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