The Evaluation of Polyphenolic Content and Antioxidant Activity of Ajuga reptans and Ajuga genevensis Extracts (Lamiaceae)

Anca TOIU; Daniela BENEDEC; Daniela HANGANU; Ilioara ONIGA

doi:10.15835/hpm.v31i1-2.14747

Anca TOIU University of Medicine and Pharmacy “Iuliu Hatieganu”
Daniela BENEDEC University of Medicine and Pharmacy “Iuliu Hatieganu”
Daniela HANGANU University of Medicine and Pharmacy “Iuliu Hatieganu”
Ilioara ONIGA University of Medicine and Pharmacy “Iuliu Hatieganu”

DOI: https://doi.org/10.15835/hpm.v31i1-2.14747

Keywords: Ajuga genevensis, Ajuga reptans, antioxidant activity, flavonoids, iridoids, polyphenols

Abstract

Several Ajuga species (Lamiaceae) are used in Romanian traditional medicine for their anti-inflammatory, wound healing and hepatoprotective properties. The aerial parts of Ajuga reptans and A. genevensis contain phenolic compounds, diterpenoids and iridoids. In the present research, the polyphenolic composition and antioxidant potential of Ajuga reptans and A. genevensis aerial parts extracts were investigated. The total polyphenolic content (TPC), flavonoids content (TFC) and iridoids content (TIC) were determined. The antioxidant capacity of Ajuga sp. extracts was measured using 2,2–diphenyl-picrylhydrazil (DPPH) and ferric-reducing antioxidant power (FRAP) methods. The results showed that the TPC ranged between 18.96-20.07 mg GAE/g, TIC from 17.93-21.17 mg AE/g, and TFC between 10.25-14.68 mg RE/g. The phenolic determinations are in accordance with the antioxidant activity, therefore a higher TPC for A. genevensis aerial parts extract is related to a higher DPPH radical scavenging activity and a higher ferric ion reducing antioxidant capacity. The results provide important preliminary information for further pharmacological research on Ajuga species aerial parts extracts.

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