Characterization of some Vitis vinifera L. Indigenous Varieties by Analysis of Leaf Photosynthetic Pigments

  • Vasile Razvan FILIMON University of Agricultural Sciences and Veterinary Medicine IaÅŸi, Romania
  • Roxana FILIMON University of Agricultural Sciences and Veterinary Medicine IaÅŸi, Romania
  • Liliana ROTARU University of Agricultural Sciences and Veterinary Medicine IaÅŸi, Romania
DOI: https://doi.org/10.15835/buasvmcn-hort:10278
Keywords: chlorophyll content meter, chlorophyll a/b ratio, chlorophyll/carotenoid ratio, spectrophotometry, Vitis vinifera L

Abstract

Chlorophyll content in leaves of 11 Vitis vinifera L. indigenous varieties (Iasi vineyard, Romania) was determined by two independent methods: spectrophotometry (extraction) and with an Opti-Sciences CCM-200 plus chlorophyll content meter (non-destructive method) with the aim of comparing the results. Also, this study was conducted to establish the values of chlorophyll a/chlorophyll b and chlorophyll/carotenoid ratio in middle leaves of vine stocks, in the phenophase of progressive growing of shoots. Area and perimeter of leaves in this phenophase were determined. Total chlorophyll concentration in leaf extract varied from 0.67±0.01 mg/g f.w. to 1.00±0.03 mg/g f.w., being highly correlated with the chlorophyll content index values (CCI) recorded by CCM-200 (R2=0.9839; p<0.001). Similar situation was identified in the case of the chlorophyll b and CCI correlation (R2=0.9638; p<0.001). Chlorophyll a/b ratio in leaf extract varied significantly depending on variety, ranging from 1.06±0.01 to 2.43±0.01, with a chlorophyll/carotenoid ratio reaching up to 3.19±0.08. Compared to spectrophotometry, CCM-200 plus affords fast, reliable, economical, non-destructive chlorophyll content measurement, however, providing no details regarding chlorophyll components and ratios.

Author Biographies

Vasile Razvan FILIMON, University of Agricultural Sciences and Veterinary Medicine IaÅŸi, Romania
DEPARTMENT V - HORTICULTURAL TECHNOLOGY - Viticulture and Ampelography - Postdoctoral researcher
Roxana FILIMON, University of Agricultural Sciences and Veterinary Medicine IaÅŸi, Romania
DEPARTMENT V - HORTICULTURAL TECHNOLOGY - Viticulture and Ampelography - PhD.
Liliana ROTARU, University of Agricultural Sciences and Veterinary Medicine IaÅŸi, Romania
DEPARTMENT V - HORTICULTURAL TECHNOLOGY - Viticulture and Ampelography - Prof. PhD.
Published
2014-11-30
Issue
Vol. 71 No. 2 (2014): Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Horticulture
Section
Horticulture and Forestry - Research Articles

 Authors who publish with this journal agree to the following terms:

a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).