Effect of Growth Regulators on In Vitro Callus Formation of Rosemary Plant (Rosmarinus officinalis L.)
Abstract
The study included the use of tissue culture technique in rosemary (Rosmarinus officinalis L.) plant propagation starting from leaf explants and the production of callus. The first experience consisted in the leaf explants sterilization using ten randomized replications of ethanol (C2H5OH), mercuric chloride (HgCl2) and sodium hypochlorite (NaOCl) respectively, on six concentrations and five treatment periods, each. The results showed that the use of sodium hypochlorite in a concentration of 0.75% for a period of 15 minutes was the most effective by giving sterile explants after two weeks of culture. After the explants sterilization, the effect of six concentrations of benzyl adenine (BA) and six concentrations of naphthalene acetic acid (NAA) and the overlap between them on the callus formation was studied. Highest callus size (mm3), callus fresh and dry weight (g) occurred at 0.2 mg/l BA and 1.5 mg/l of NAA.
Authors who publish with this journal agree to the following terms:
a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
