Aspects Regarding the In vitro Propagation of ‘Royal Gala` Apple Cultivar
Abstract
Apple cultivar ‘Royal Gala` was multiplied in vitro on starch-gelled MS media supplemented with 0.7 mg/l BAP, which provided intense proliferation of axillary shoots. In order to test the influence of gelling agents upon proliferation rate in this apple cultivar, modified MS media were used, supplemented with 0.7 mg/l BAP and five types gelling agents: fibrous agar, 6.8 g/l; Phytagel + starch, respectively 0.5 + 50 g/l; guar gum, 20 g/l; Psyllium husk, 15 g/l; Phytagel at 2.2 g/l. The highest proliferation rate, 13.44 ± 1.22 was obtained when gelling the media with the mixture of Phytagel + starch. By using ex vitro rooting and acclimatization in floating perlite, the rooting percentages were between 99.45% and 93.92%, depending on the culture media used in the multiplication stage, from which the axillary shoots were harvested.
Authors who publish with this journal agree to the following terms:
a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
