Patentable Formula Based on Essential Oils with a Protective Effect Against Rhizobium Vitis

Abstract

The crown gall of grapevines is an economic importance bacteriosis caused by Rhizobium vitis strains. Five essential oils (EO’s) were tested against the autochthonous 2btm strain of R. vitis for their capacity to inhibit the phytopathogen growth, by in vitro and in planta tests. The measurements regarding in vitro inhibition zone were performed. Subsequently, the ability of patentable formula based on essential oils to prevent/reduce tumor formation in tomato and vine plants has been tested. After 30 days for tomato plants, the evaluation of disease incidence and effectiveness of treatments applied at two points in time (T₀ and T₃₀), were determined. In vine plants, tumorigenesis is initiated up to 9 months after inoculation, so the results are being processed. Efficacy values of 94.7% at both T₀ and T₃₀   and, a very low disease incidence of 5% compared to positive control were recorded. Also, the tumor of EO’s treated tomato plants were smaller in size and did not form a complete ring as positive control plants. The use of patentable formula as prevented treatment, reduced the number of plants developing crown gall symptoms and tumor size compared to positive control plants.