Breeding a New Apple Hybrid Population with the Vf Gene Through Marker-Assisted Selection

  • Georgeta Maria BIVOLARIU (GUZU) Fruit Research & Development Station Bistrița, 3 Drumul Dumitrei Nou Street, Bistrița, 420105, Romania; Faculty of Horticulture and Bussines in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur Street, Cluj-Napoca, 400372, Romania
  • Ioan ZAGRAI Fruit Research & Development Station Bistrița, 3 Drumul Dumitrei Nou Street, Bistrița, 420105, Romania
  • Claudiu MOLDOVAN Fruit Research & Development Station Bistrița, 3 Drumul Dumitrei Nou Street, Bistrița, 420105, Romania
  • Smaranda Doina ROȘU MAREȘ Fruit Research & Development Station Bistrița, 3 Drumul Dumitrei Nou Street, Bistrița, 420105, Romania
  • Anca Maria CHIOREAN Fruit Research & Development Station Bistrița, 3 Drumul Dumitrei Nou Street, Bistrița, 420105, Romania
  • Luminița Antonela ZAGRAI Fruit Research & Development Station Bistrița, 3 Drumul Dumitrei Nou Street, Bistrița, 420105, Romania
  • Mirela Irina CORDEA Faculty of Horticulture and Bussines in Rural Development, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, 3-5 Mănăștur Street, Cluj-Napoca, 400372, Romania
DOI: https://doi.org/10.15835/buasvmcn-hort:2024.0024
Keywords: Malus domestica; marker; monogenic resistance; scab; Vf gene.

Abstract

Apple scab, caused by Venturia inaequalis, is one of the most damaging pathogens affecting apple species. Cross combinations were made between the Salva cv. (female parent), a valuable local cultivar known for its fruit quality and used as a donor of the Vf resistance gene, and Jonathan (male parent), a variety widely cultivated but susceptible to scab. The Vf gene was first identified in Malus floribunda Clone 821, which was subsequently transferred to commercial varieties through various breeding programs. To confirm the presence of the Vf gene, the progeny from this cross was tested using Marker-Assisted Selection (MAS) with one dominant primer pair (AM19) and two codominant primers (AL07 and VFC) to distinguish between homozygous and heterozygous genotypes. From the crossing, 67 hybrids were obtained, of which 35.8% (24 hybrids) were classified as resistant (heterozygous - Vfvf), while 64.2% (43 hybrids) were classified as susceptible (recessive homozygotes - vfvf). This population of hybrids resistance to Venturia inaequalis represents a good starting point in obtaining cultivars with resistance to scab.

Published
2024-11-15
Issue
Vol. 81 No. 2 (2024): BULLETIN OF UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE CLUJ-NAPOCA. HORTICULTURE
Section
Research Articles

 Authors who publish with this journal agree to the following terms:

a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).