TISSUE CULTURE AND EX-VITRO ACCLIMATION OF RHODODENDRON sp.

Clapa Doina, Al. Fira

Abstract


The majority of Rhododendron species reproduce by seeds, but the cultured varieties the production of planting material is done vegetatively by greenwood cuttings or by grafting. Another, much faster and more economical propagation method is propagation by tissue culture. In this paper aspects regarding the micropropagation of this species are presented, respectively, initiation, multiplication and acclimation. For initiation two variants of media were used: V1-Murashige & Skoog and V2-Woody Plant Medium (McCown). The biologic material used for initiation is represented by 2-5 cm long young shoots taken from plants isolated in the greenhouse, juvenilized by repeated prunings. For multiplication, two variants of medium were used, also, V1’- Woody Plant Medium (McCown)+ 2 mg/l Zeatin and V2’- Woody Plant Medium (McCown)+ 5 mg/l 2-Ip.

Keywords


Rhododendron; tissue culture; initiation; multiplication; acclimation

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DOI: http://dx.doi.org/10.15835/buasvmcn-hort:1899


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