In Vitro Propagation of the Thornless Blackberry Cultivar ’Loch Ness’

  • Alexandru FIRA Fruit Research Station Cluj, 5 Horticultorilor Street, 400457 Cluj-Napoca, Cluj, Romania
  • Doina CLAPA Fruit Research Station Cluj, 5 Horticultorilor Street, 400457 Cluj-Napoca, Cluj, Romania
  • Elena RAKOSY -TICAN Babes-Bolyai University, 1 Mihail Kogalniceanu street, Cluj-Napoca, Cluj, Romania

Abstract

Our paper presents aspects regarding the in vitro multiplication rates, the fresh biomass of the resulted plantlets, aspects regarding the incubation of the cultures in the growth room and the rooting and acclimation of the newly formed shoots in the thornless blackberry cultivar ‟Loch Ness‟. The nutritive medium had, as components Murashige & Skoog 1962 (MS) salts, Myo-Inositol at 100 mg/l, Vitamins B1- 1 mg/l, B6 - 0.5 mg/l, Nicotinic Acid - 0.5 mg/l and, as growth regulator, 6-benzylaminopurine at 0.5 mg/l. The rooting of the shoots that resulted from the multiplication medium was done ex vitro in the same phase together with acclimation, in floatation hydroculture as well as in solid substrate (acid peat + perlite in 1:1 volume to volume ratio. Ex vitro rooting and acclimation in float hydroculture in open air and the subsequent growth and development of the acclimated plants in hydroponics fertilized with 2 g/l Ferticare were also investigated.