PCR Protocol Optimization for Genetic Diversity Assessment and Molecular Characterization of Sour Cherry Cultivars
Abstract
Sour cherry is an economical important tetraploid species in Europe. Use of SimpleSequence Repeats (SSRs) as descriptors to characterize and differentiate a set of 15 sour cherryvarieties from three National Collections from Romania, represented by selections from local varietiesand hybrids from our country and foreign origin was taken as a leading case study for plant varietyprotection purposes. A set of sixteen SSR markers taken from the literature which was used for geneticdiversity in different genus of Prunus such as sweet cherry, sour cherry, peach, apricot. The PCRamplification protocol and annealing temperature for each primer pair was optimized for use in sourcherry. In these partial results, only for five (25%) primer pairs the optimum annealing temperature toamplify the sour cherry varieties was found. Similarly, 63°C and 66.2°C were found optimum forprimer pairs pchgms1 and UDP96-003, only the last one from all primer pairs was polymorphicbetween varieties. For primer pairs pchgms2 the annealing temperature was 64°C and 66°C; 67°C and69°C for UDP96-001; 61.3°C for UDP97-402. No more then four fragments were amplified for eachvarieties/primer pair combination. From these results we conclude the transferability and applicabilityof SSR markers for genotyping and phylogenetic studies in the genus Prunus. Authors who publish with this journal agree to the following terms:
a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).