CORRELATION BETWEEN -METHYL-ACYL CO-A RACEMASE (AMACR) AND PROSTATE SPECIFIC ANTIGEN (PSA) LEVELS IN BLOOD SAMPLES OF PROSTATE CANCER PATIENTS

Raluca Dumache "Victor Babes’’ University of Medicine and Pharmacy, Biochemistry Department, Eftimie Murgu Plaza,no2,Timisoara 300041
F. Miclea ‘’Victor Babes’’ University of Medicine and Pharmacy, Urology Department,
B. Bumbăcilă "Victor Babes’’ University of Medicine and Pharmacy, Biochemistry Department, Eftimie Murgu Plaza,no2,Timisoara 300041
Maria Puiu ‘’ Victor Babes’’ University of Medicine and Pharmacy, Medical Genetics Department,

DOI: https://doi.org/10.15835/buasvmcn-vm:65:1:1239

Keywords: ?-methyl-acyl Co-A racemase (AMACR), prostate specific antigen (PSA), RT-PCR, prostate cancer, benign prostatic hyperplasia (BPH).

Abstract

In our study we want to demonstrate that -methylacyl Co-A racemase (AMACR), has an highly sensitivity and specificity for the prostatic tissue, and it can be used in combination with PSA (prostate specific antigen) for the detection of prostate cancer.AMACR is a peroxisomal and mitochondrial enzyme involved in the -oxidation of branched fatty acids. The gene encoding AMACR is over-expressed in prostatic tissue. It is well known that the main source of branched fatty acids are the dairy products and red meat pork and beef, the consumption of which has been associated with an increased risk for prostate cancer. For our study we evaluated the AMACR gene expression in 82 patients aged 50 to 80, with total PSA values in a range of 3 to 46 ng /ml. From the 82 patients, 53 were diagnosed positive for prostate cancer and they had PSA values greater than 17ng/ml. In these 53 men, we found using RT- PCR techniques , that they had an over expressed gene for AMACR. The other 29 with benign prostatic hyperplasia, with PSA values smaller than 10 ng/ml, had normal expression of the AMACR gene. According to these results we propose the use of AMACR as an important novel biomarker for the prostate cancer in combination with the PSA and the histological exam.

Published

2008-11-21

Issue

Vol. 65 No. 1 (2008)

Section

Research Articles

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