THE STUDY OF THE VASCULARISATION IN BITCH MAMMARY TUMORS
Abstract
The purpose of the study is to see if there could be made an association between mammary tumor angiogenesis and malignancy degree. Our study made a correlation between classical aspects that denote mammary tumor malignancy in bitches, and intratumor vascularity. There were realized a quantification of neoformed microvessels on microscopic field, and also determined the perimeter and aria of sanguine or lymphatic microvessels, utilizing an Olympus image analyzing system. Intratumor micro vessel density appreciation was realized using LSAB immunohistochemistry, microvessels being labeled with CD31 marker (Dako). The analyzed microscopic images were magnified of 200x, and vascular perimeter and aria were determined by Olympus DP Soft image analyzing program. The obtained results had been expressed in μm (perimeter) respective μm2 and percentage (area). The results had been statistically interpreted, to follow up if the studied parameters could be used as malignancy parameters in bitch mammary tumors
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