Advanced Techniques of Bovine Semen Analysis

  • Mihai CENARIU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Emoke PALL University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Mihai BORZAN University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Liviu BOGDAN University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
  • Ioan GROZA University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Keywords: bull semen, CASA, fertility, FACS, light microscopy

Abstract

The aim of the study was to assess bull semen fertility parameters using the classical techniques of sperm quality evaluation (density, motility, viability, and morphology, evaluated by light microscopy, in addition to concentration, evaluated via the hemocytometer and microspermatocrit), as well as advanced techniques, like computer assisted sperm analysis (CASA) and flow cytometry. Results obtained for classical techniques were comparable to those obtained by automated methods, without significant differences between parameters. The classical methods were inexpensive but required more time and attention, while the operator’s experience was a key element for accurate assessment of sperm parameters. The advanced techniques were fast and objective, but required expensive equipment and dedicated personnel, with proper training in the field. Therefore, classical techniques are suitable for clinics where occasional evaluation of bulls’ fertility parameters is performed, while the advanced methods should be implemented in semen companies, as well as in fertility clinics and research laboratories.

Author Biographies

Mihai CENARIU, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Animal Reproduction and Reproductive Pathology
Emoke PALL, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Animal Reproduction and Reproductive Pathology
Mihai BORZAN, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Animal Breeding
Liviu BOGDAN, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Animal Reproduction and Reproductive Pathology
Ioan GROZA, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca
Department of Animal Reproduction and Reproductive Pathology
Published
2018-05-19