Bactericidal Capacity of Serum and Enhancement of Specific Cell-Mediated Immunity Subsequent to Hipopphae Rhamnoides Treatment in Pigs

  • Laura Andreea RUSU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca
  • Aurel VASIU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj
  • Emoke PALL University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj
  • Diana Ioana OLAH University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj
  • Constantin CERBU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj
  • Carmen Dana ŞANDRU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj
  • Gheorghița DUCA University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj
  • Marina SPÎNU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Abstract

Low-input farming subject’s swine to increased immune stress and subsequent infectious risk, which could be prevented by phytotherapy. Hypopphae rhamnoides is a widespread shrub in both Europe and Asia. The research aimed at investigating the influence of a whole fruit extract on antibacterial effect of the serum in extensively raised, two batches, sea buckthorn in vivo treated/untreated pigs. Scavenging effect over DPPH was used to estimate the antioxidant potential of the sea buckthorn commercial syrup (Steaua Divina©). Minimal inhibiting (MIC) and minimal bactericidal (MBC) capacity of the serum were tested and spectrophotometrically measured against Shigella spp., Kitococcus sedentarius, Staphylococcus werneri and Staphylococcus sciuri. The antioxidant activity of the syrup was intermediate (54.65 %). Sera from treated pigs had bactericidal and bacteriostatic effect on Shigella spp. and S. sciuri, respectively. Shigella spp. growth was inhibited at serum dilutions of ½-1/32 while much lower ½-1/4 dilutions were active against S. sciuri. In untreated pigs, the sera were inefficient in either stopping or totally inhibiting bacterial growth. The MBC and MIC values obtained for the sera of pigs treated with Hippophae rhamnoides syrup supported the positive effect of the in vivo treatment inducive of potential protection against bacterial diseases.

Author Biographies

Laura Andreea RUSU, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca

Faculty of Veterinary Medicine

Aurel VASIU, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Faculty of Veterinary Medicine

Emoke PALL, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Faculty of Veterinary Medicine

Diana Ioana OLAH, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Faculty of Veterinary Medicine

Constantin CERBU, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Faculty of Veterinary Medicine

Gheorghița DUCA, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Faculty of Veterinary Medicine

Marina SPÎNU, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Mănăștur St. no 3-5, 400372, Cluj-Napoca, Cluj

Faculty of Veterinary Medicine

Published
2022-11-14