THE EFFECT OF PHENOL, USED IN BIOLOGICAL PRODUCTS, ON PK- 15 CELL CULTURE. A NOTE.
Abstract
Phenol was added in cultures of PK-15 cell line, at cell passage and on formed monolayer, in concentrations of 1/100, 1/500, 1/1000, 1/5000, 1/10000,1/15000 and 1/20000, in medium (g/ml). The cultures were incubated at 37,5°C, in 5% CO2, up to 24 hours. Untreated cultures served as control. With the decreasing concentration, the addition of Phenol at cell passage has totally or partially blocked the monolayer formation, about 90% formed monolayer being observed at concentration of 1/20000. Added on formed monolayer, Phenol has produced partial detachment of cells up to the concentration of 1/1000. At 1/5000, the monolayer remained relative normal. According to experimental results, Phenol elicits a strong cytotoxic effect on PK-15 cell culture
Authors who publish with this journal agree to the following terms:a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
