HYPERIMMUNISATION OF RABBITS WITH ULTRASOUND DISINTEGRATED BORDETELLA BRONCHISEPTICA AND CONTROL OF ANTIBODY SYNTESIS TROUGH DOUBLE DIFFUSION AGAR GEL

Gh Răpuntean University of Agricultural Sciences and Veterinary Medicine, Faculty of Veterinary Medicine 3-5 Mănăştur Street, 400372, Cluj-Napoca
A. L. Dánes

DOI: https://doi.org/10.15835/buasvmcn-vm:64:1-2:2407

Keywords: Bordetella bronchiseptica, ultrasounds, disintegration, hyperimmunisation rabbits

Abstract

Two rabbits were hyperimmunisated with an ultrasound lysate of a pig origin B. bronchiseptica and one rabbit was hyperimmunisated with an ultrasound lysate of B. bronchiseptica dog strain origin. Each rabbit was inoculated three times. Blood samples were collected before the hyperimmunisation begun and after each inoculation. Antibody production during immunization was controlled using the method of double diffusion in agar gel. After the first inoculation only 2 of 3 rabbits responded with antibody production detectable with this method, but after the third inoculation all the rabbits responded with antibody production at least against three antigenic compounds of the ultrasound disintegrated B.bronchiseptica. Serum samples obtained from each rabbit after the third antigen inoculation were tested against both antigen solutions. These tests proved that all rabbits produced antibodies against the same three antigenic compounds of B. bronchiseptica.

Published

2008-12-02

Issue

Vol. 64 No. 1-2 (2007)

Section

Research Articles

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