TESTING COMPATIBILITY AND EVALUATING THE RISK OF BLOOD TRANSFUSIONS IN A SAMPLE OF DOGS THAT UNDERWENT INTENSIVE THERAPY
Abstract
Recurring to blood; transfusion as a procedure in intensive therapy was based on the assessment of compatibility and transfusion risks; by testing blood groups performing major Crossmatch compatibility test; in a heterogeneous population of dogs (n = 139) from the central Transylvania. Compatibility tests were conducted in the laboratory of Physiology FMV Cluj-Napoca; and the blood transfusions Emergency hospital of the same institution. The blood types were determined through the tube agglutination method; with SHIGETA kits; containing 4 types of monoclonal antibodies. There have been verified also 3 variants major Crossmatch; one of which; in consisting of rapid testing on glass slate; and was used alongside blood typing in assessing the compatibility of 22 patients that underwent and transfusions. The average values (+++; ++++) of increased intensity of the agglutination reaction have shown that monoclonal SHIGETA antibodies performed a good detection of each erythrocyte antigen type for this antigenic system. In the frequency evolution of the blood phenogroup; the major percentage possessed the B antigen: 1.1B (45.32%); 1 (-) B (23.74%) and 1.2B (20.86%). These were predominant in all breeds investigated; similar percentages being registered for 1(-) B and 1.1B in German shepherd and English bulldog. Major percentage of 1.1B was registered in other breeds. Analysis of the overall compatibility tests indicated German Shepard bread as an important source of potential donors. Confirmation of this was given by increased proportion of German shepherds 1 (-) B positive (50%); as a result of the correlation between 2 antigenic systems (SHIGETA and DEA) these have proven to be DEA 1 negative. Results of preliminary tests verify the blood compatibility based on blood type and Crossmatch were negative in the case of a family of 4 German shepherd females 1 (-) B positive. Using these tests in the case of patients subjected to blood transfusions contributed to their recovery in the proportion of 90%. On other two patients the very serious development has been associated with fatal hemolysis after the third; respectively the fourth transfusion with blood from compatible a donors
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