Comparative Study of S100 Marker Expression by Immunohistochemistry in Canine Melanic Tumors - Association with Macroscopic and Microscopic Aspects
Abstract
We evaluated the expression of S100 ,compared routine immunostaining of 9 clinical specimens from melanoma cases,1 metastatic melanoma and melanocytoma using with staining for S100 as part of a diagnostic panel. All cases are positive with S100 (11/11-100%) Classification of intense of S100:9/11 (81.81%) had 51–80% positive cells, and 2/11 (18.19 %) had more than 80% positive cells. In melanoma cases the intensity of staining was high in 2/9 (33.33 %), over moderate in 7/9(77.77%), but, the staining was over moderate in the metastasis (79.84%) and in melanocytoma (54.94%). Nuclear staining was visible in 2 cases, and was staining visible in some cells and invisible in others in 5 cases and was no visible in 4 cases . All tumor cell types were reactivity for S100 .The staining in the majority of cases was heterogeneous in 8 cases, while 3 cases were homogenous. Epithelioid cells were a tendency for staining of moderate to intense between (63.83% to 98.1%). mixed epithelioid and spindle cells were a tendency for staining of moderate grade between (56.66% to 73.66%) Spindle cells were staining of moderate in (2/2) one of them is melanocytoma (54.06% - 54.94%) One balloon cells type melanoma with moderate. High percentages of marker stain accompany with medium of number of mitosis. Discussion: All cases were positive with S100.The percentages of marker stain were between ( 54.06% - 98.1%). All cases were between 3-4 grade that indicate to tend cells to stain .Melanoma cases distributed between moderate and intense stain while melanocytoma was moderate. Capable to stain nuclear with S100 was weak .The staining in the majority of cases was heterogeneous. Epithelioid cells were a tendency for staining of moderate to intense that indicate to capability these cells to stain with S100, Mixed epithelioid and spindle cells type,the balloon cells type and spindle cell type were a tendency for staining of moderate all cases tumoural cells were reacted diffusely in the cytoplasm.
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