Immunotoxic Action of Ochratoxine A on Lymphocytes from Lymphoid Tissues Associated to Gut Mucosa in Chickens
Abstract
Investigation were conducted on 2 groups, each of 15 broiler chickens. Ochratoxine A(OTA) was given orally, in sunflower oil suspension, daily , for 21 days in doses of 50 μg/kg g. Control group (of 15 chickens) received only sunflower oil, 5 chickens from each group were killed after 7,14 and 21st day of the experiment. Gut mucosa samples were prevealed from duodenum, jejunum and caecal tonsils. All samples were prepared for paraphin embeding and stained with: monoclonal antibody Tcr1, TCR2, CD3 and CD8. In duodenum, the cryptae from lamina propria showed numerous CD3, with nucleus rich in euchromatine, with 3-4 nucleoli. The enterocytes had a large nucleus rich in euchromatine, with 3-4 nucleoli. Progressively with the age and OTA dosis the lipidic droplets from the axis of the villi and proportion of degenerated enterocytes are increasing and simple epithelium is replaced by stratified epithelium, with many immature cells. In jejunum, the cryptae from lamina propria showed numerous CD8. The coecal tonsils, after 14 days of OTA poisoning showed lamina propria populated by small lymph cells TCR1. Some of the lymph cells showed nuclear lesions like: cariorhexis, cariolysis and other show apoptotic aspects. Into the lymphoid agglomerations some cells with nuclei rich in euchromatine, with 4-5 nucleoli were observed
Authors who publish with this journal agree to the following terms:a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
