Optimization Method For Biomolecular Identification of Muscle Larva of Trichinella species
Abstract
Trichinellosis is a food-borne parasitic zoonosis with a yearly incidence of about 10,000 clinical cases worldwide. It is one of the most serious zoonotic diseases in Romania with more than 28,000 human cases reported over the last 25 years. In this context Romania remains the country with the highest infestation with Trichinella from the word. The aim of the paper it is to identify the simple repetitive sequences from Trichinella genus through a biomolecular analysis of isolates. After conducting the DNA extraction from 50-100 muscle larvae of the six selective strains (jackal, wolf 1, wolf 4, 7.97, 7.42 and 7.29) using the “ QIAamp DNA” (Qiagen, Germany) the amplification with ESV primers (forward and reverse) using the “mi-Taq Mix Kit ”(Metabion, Germany) followed, obtaining in this manner a positive amplification for all the six strains taken into study, revealing also the method's efficiency in the biomolecular identification of Trichinella strains. The goal of this research, in perspective, is to improve the DNA extraction from one LM, in order to reduce the chemical and biological reagents. The positive amplification of the genomic DNA from one larva may have favorable repercussions on the genetic variability analysis in the population of Trichinella studied, which can be applied on various individuals belonging to the same population.
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