Researches Regarding the Influence of Apitherapy Diet on Leukocyte Formula in Wistar Rats with Experimentally CCl4 Induced Liver Disease

  • Călin V. ANDRIŢOIU Department of Physical Chemistry, “Gheorghe Asachi” Technical University, Prof. dr. docent Dimitrie Mangeron 73 Street, Iaşi, România
  • Vasile ANDRIŢOIU Department of Cellular and Molecular Biology, University of Medicine and Pharmacy ,,Gr. T. Popa”, Universitatii 16 Street, Iaşi, Romania
  • Anca I. PRISĂCARU Department of Natural and Synthetic Polymers, “Gheorghe Asachi” Technical University, Prof. dr. docent Dimitrie Mangeron 73 Street, Iaşi, România
  • Carmen E. COTRUTZ Department of Cellular and Molecular Biology, University of Medicine and Pharmacy ,,Gr. T. Popa”, Universitatii 16 Street, Iaşi, Romania
  • Tudor PETREUŞ Department of Cellular and Molecular Biology, University of Medicine and Pharmacy ,,Gr. T. Popa”, Universitatii 16 Street, Iaşi, Romania
  • Ionel M. POPA Department of Physical Chemistry, „Gheorghe Asachi” Technical University, Prof. dr. docent Dimitrie Mangeron 73 Street, Iaşi, România,
DOI: https://doi.org/10.15835/buasvmcn-vm:1:68:6721

Abstract

The purpose of this experiment is to evaluate the influence of apitherapy diet on CCl4 induced hepatopathy in laboratory animals, by the means of leukocyte formula. The experiment was unfolded on six groups of Wistar rats: control group standard food (group I), control group apitherapy diet (group II), control group apitherapy diet + royal jelly (group III), CCl4 group (group IV), group CCl4 + apitherapy diet (group V), group CCl4 + apitherapy diet + royal jelly (group VI). The animals were handled under general anesthesia with thiopental. Hepatic lesion was induced by intraperitoneal injection of CCl4 (dissolved in paraffin oil, 10% solution). Hepatoprotection was achieved with apitherapy products (Apiregya, ApiImunomod, ApiImunostim, ApiImunostim Forte). Determination of the leukocyte formula was achieved by using an automatic analyzer and commercial kits. Administration of apitherapy diet and respectively of apitherapy diet and royal jelly in laboratory animals with CCl4 induced hepatopathy improves the values of parameters from the leukocyte formula when compared with CCl4 group.
Published
2011-10-31
Issue
Vol. 1 No. 68 (2011): Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
Section
Research Articles
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