Molecular Detection of Pasteurella multocida Strains from Oral Swabs in Pets

  • Simona IVANA “Asclepius” Research Center, 93, Gheorghe Şerban street, district 2, Bucharest Corresponding author: Simona Ivana, Faculty of Veterinary Medicine, 105, Splaiul Independentei, 050097, Bucharest, Romania
  • Viorica CHIURCIU Romvac Company, Voluntari, Romania
  • Dana Magdalena CAPLAN "Cantacuzino" National Researches-Development Institute for Microbiology-Immunology, 104, Splaiul Independentei, district 5, Bucharest
  • Camelia BALA Bucharest University, 36-46, Bd. M. Kogălniceanu, district 5, 050107, Bucharest
  • Lucian IONIŢĂ Faculty of Veterinary Medicine, University of Agronomical Science and Veterinary medicine Bucharest, 105, Splaiul Independentei, 050097, Bucharest, Romania
  • Carmen IONIŢĂ Faculty of Veterinary Medicine, University of Agronomical Science and Veterinary medicine Bucharest, 105, Splaiul Independentei, 050097, Bucharest, Romania
  • Iudith IPATE Acad. David Davidescu Studies and Researches Center for Agriculture and Forestry Biodiversity, 13, 13 Septembrie bd., district 5, Bucharest, Romania
  • Cristina PÂRVU “Carol Davila” Medicine and Pharmacy University, Bucharest
  • Alexandru POPESCU “Asclepius” Research Center, 93, Gheorghe Şerban street, district 2, Bucharest Corresponding author: Simona Ivana, Faculty of Veterinary Medicine, 105, Splaiul Independentei, 050097, Bucharest, Romania
DOI: https://doi.org/10.15835/buasvmcn-vm:1:68:6762

Abstract

The molecular detection by means of the polymerase chain reaction (PCR) represents a much more sensitive, specific and fast detection method of Pasteurella and confirms the infection. Although the isolation and identification of the pathogenic agent represent the most reliable method of demonstration of its existence, the results of the bacteriologic examination are frequently false negative, owing to the fact that P. multodica is either rather easily destroyed during the transportation or eliminated from the culture medium by the bacteriological suprainfection germs (nasal flora or contaminants).Pasteurellae were identified both in clinically healthy cats and in ill cats. These dates suggest that the contaminated cats, regardless of their clinical state, may transmit de bacteria to humans by bite or scratch. In this work, cats kept as pets from Bucharest were studied with suspect to their contamination with Pasteurella. Of 44 samples taken from the oral cavity of cats, 22 Pasteurella spp. strains could be isolated, 18 of which belonged to the Pasteurella multocida. The organisms were isolated both from healthy and from infected cats.
Published
2011-11-01
Issue
Vol. 1 No. 68 (2011): Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
Section
Research Articles
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