Immunohistochemical Quantification of the Tumor Necrosis Factor (TNF) Receptor II Expression in the Hepatic Tissue after Systemic Administration of the DNA-SWCNT

  • Flaviu TABARAN University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania
  • Cornel CĂTOI University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania
  • Simona CLICHICI University of Medicine and Pharmacy “Iuliu Hateganu”, Str. Emil Isac No. 13, code 400372 Cluj-Napoca, Romania
  • Teodora MOCANU University of Medicine and Pharmacy “Iuliu Hateganu”, Str. Emil Isac No. 13, code 400372 Cluj-Napoca, Romania
  • Alexandru BIRIS National Institute for Research and Development of Isotopic and Molecular Technologies, 65 – 103 Donath str., 400923, Cluj – Napoca, Romania
  • Stefania SIMON National Institute for Research and Development of Isotopic and Molecular Technologies, 65 – 103 Donath str., 400923, Cluj – Napoca, Romania
  • Andras NAGY University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania
  • Marian TAULESCU University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania
  • Pompei BOLFĂ University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Romania
DOI: https://doi.org/10.15835/buasvmcn-vm:1:68:6828

Abstract

The Tumor Necrosis Factor is a key mediator in hepatic inflammatory response during acute exposure to xenobiotics. The cellular effects of TNF are mediated via two cell surface receptors, TNF receptor 1 and TNF receptor 2. The purpose of this study was to examine the expression and to identify the cellular localization of TNFR2 in hepatic tissue by immunohistochemistry after systemic administration of the SWCNT. In the same time the cellular infiltration and the weight of the liver was correlated with the TNF alpha receptor 2 expression. Mice were exposed intraperitoneally (ip) to either vehicle, phosphate-buffered saline (PBS), or SWCNT-DNA (1.5ml, 2.925 mg/kg) for 48 h. The hepatic response associated with SWCNT systemic administration was characterized by increased expression of the TNF receptor II especially in the hepatocytes from the centrilobular and midzonal areas of hepatic lobule. This induced elevation of the TNF receptor II is not followed by hepatic necrosis, inflammatory infiltration or significant changes in liver weight.
Published
2011-11-01
Issue
Vol. 1 No. 68 (2011): Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine
Section
Research Articles
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