Haemoparasite Prevalence, Genetic Diversity of TLR2B Gene and Relationship with Haematological Parameters in Chicken

  • Babatunde Moses ILORI Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State
  • Matthew Adekunle ADELEKE School of Life Sciences, University of KwaZulu Natal (Westville Campus), P/Bag X54001, Durban 4000
  • Adebisi Adegboyega ADELEYE Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State
  • David Oluwafemi OGUNTADE Ambrose Alli University, Ekpoma
  • Samuel Olutunde DUROSARO Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State
  • Olufemi Adekunle OSONOWO Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State
  • Michael Irewole TAKEET Federal University of Agriculture, P.M.B. 2240, Abeokuta, Ogun State
  • Kayode AKANO Michael Otedola College of Primary Education, Noforija, Epe, Lagos State
  • Henry Temitope Akinloluwa OJOAWO Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State
  • Michael Ohiokhuaobo OZOJE Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State
DOI: https://doi.org/10.15835/buasvmcn-asb:2022.0002

Abstract

Haemoparasite constitute a major challenge in native chicken production in Africa. This study determines the genetic diversity and the effect of TLR2B gene polymorphism on haemoparasite and haematology of the chickens based on genotype and sex. 600 chickens of 25 weeks old consisting of Naked neck (NN), Normal feather (NF), and the Frizzle feather (FF) reared in battery cage-system were sampled for blood and analyzed for haematology, parasite occurrence and load, polymerase chain reaction, and gene sequencing. Polymorphisms were detected and their effect on haematology was determined. Results showed the occurrence of Plasmodium gallinacieum, Trypanosoma brucei, and Leucocytozoon schoutedeni with NF having the highest occurrence followed by NN and FF chicken genotype. There was a significant (P <0.05) effect of genotype and sex on haematology. Seven of the eight polymorphism detected were singleton and found only in NF while parsimonious 656GA was detected in all the chicken genotypes with no relationship with haematology and haemoparasite. NF had the highest nucleotide (0.00114) and haplotype diversity (0.584). The study revealed the occurrence of genetic variation in TLR2B gene, haematology, and haemoparasite in FUNAAB Alpha chickens which could provide baseline information in future breeding programmes of the chicken for the tropical environment.

Author Biographies

Babatunde Moses ILORI, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State

Department of Animal Breeding and Genetics

Matthew Adekunle ADELEKE, School of Life Sciences, University of KwaZulu Natal (Westville Campus), P/Bag X54001, Durban 4000

Discipline of Genetics

Adebisi Adegboyega ADELEYE, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State

Department of Animal Breeding and Genetics

David Oluwafemi OGUNTADE, Ambrose Alli University, Ekpoma

Department of Animal Science

Samuel Olutunde DUROSARO, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State

Department of Animal Breeding and Genetics

Olufemi Adekunle OSONOWO, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State

Department of Animal Breeding and Genetics

Michael Irewole TAKEET, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Ogun State

Department of Veterinary Microbiology and Parasitology

Kayode AKANO, Michael Otedola College of Primary Education, Noforija, Epe, Lagos State

Department of Agricultural Education

Henry Temitope Akinloluwa OJOAWO, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State

Department of Animal Breeding and Genetics

Michael Ohiokhuaobo OZOJE, Federal University of Agriculture, Abeokuta, P.M.B. 2240, Ogun State

Department of Animal Breeding and Genetics

Published
2022-11-14
Issue
Vol. 79 No. 2 (2022): BULLETIN OF UNIVERSITY OF AGRICULTURAL SCIENCES AND VETERINARY MEDICINE CLUJ-NAPOCA. ANIMAL SCIENCE AND BIOTECHNOLOGIES
Section
Research Articles
 Authors who publish with this journal agree to the following terms:

a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).