THE OVINE LEPTIN GENE ISOLATION AND AMPLIFFICATION

CLAUDIA TEREZIA SOCOL, A. VLAIC, VIORICA FLORICA COSIER

Abstract


Leptin is a 16kDa nonglycosylated hormone synthesized and secreted by white adipose tissue. which acts primarily in the hypothalamus to reduce food intake and body weight. which is involved in important metabolic pathways. the reproduction activity. the pathology of some systems from the animal and human organism (the circulator system. the bony system. diseases of the genital area. obesity). The isolation of the leptin gene and its amplification is one of the first steps in the laboratory experiments of obtaining the recombinant leptin. In this paper it is described an optimized efficient method for the isolation of the ovine leptin gene. having as a starting material the RNA extracted from the ovine adipose tissue. The main advantage of using the recombinant DNA technology for obtaining recombinant proteins is the possibility of performing this synthesis process to a larger scale. realizing in this way a space and a time economy and also an important financial achievement. eliminating the classical methods of extraction of different hormones or proteins from animal tissues. avoiding the risk of allergical and immunological reactions. the contamination with viruses.

Keywords


leptin gene; adipose tissue; RNA isolation; RT-PCR; agarose gel; electrophoresis

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DOI: http://dx.doi.org/10.15835/buasvmcn-asb:64:1-2:2186




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