RAPD Analysis of Romanian Silkworm Genetic Biodiversity (Races X Hybrids)

EMILIA FURDUI University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,
LIVIU-ALEXANDRU MARGHITAS University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,
DAN DEZMIREAN University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,
IOAN PASCA University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,
CRISTIAN COROIAN University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,

DOI: https://doi.org/10.15835/buasvmcn-asb:66:1-2:3396

Keywords: silkworm, hybrids, phylogenetic relationships, Bombyx mori, RAPD

Abstract

Genetic differences are followed in the present study between different Bombyx mori genotypes from Romania, with the help of RAPD techniques. DNA extraction was made by using several working protocols: classic (Suzuki et al., 1972) and 2 rapid ones (extraction kits from Promega: WGDP - Wizard Genomic DNA Purification Kit and WMDP - Wizard Maxwell DNA Purification Kit). 44 primers were used and 34 have generated polymorphic bands. Amplification products resulted by RAPD are different due to the genotype analyzed lines structures, resulting significant differences between strains and hybrids. Bombyx mori genome analysis was initiated having the purpose to generate genetic maps for the characterization of genetic diversity using different marker systems: RFLP, RAPD.

Author Biographies

EMILIA FURDUI, University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,

BIOTECHNOLOGIES

LIVIU-ALEXANDRU MARGHITAS, University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,

BIOTECHNOLOGIES

DAN DEZMIREAN, University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,

BIOTECHNOLOGIES

IOAN PASCA, University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,

BIOTECHNOLOGIES

CRISTIAN COROIAN, University of Agricultural Science and Veterinary Medicine, Faculty of Animal Science and Biotechnologies, 3-5 M?n??tur Street, 400372 Cluj-Napoca,

BIOTECHNOLOGIES

Issue

Vol. 66 No. 1-2 (2009): Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Animal Science and Biotechnologies

Section

Research Articles

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