Preliminary Results on Isolation and Cloning of Gene Fragments Involved in Potato Resistance to Phytophthora infestans
Abstract
Late blight is the most devastating disease of potato (Solanum tuberosum) worldwide. It is caused by the oomycete Phytophthora infestans. Wild Solanum species are a rich source of resistance genes to Phytphthora infestans. Solanum demissum is the most studied and used potato in breeding programs. 11 resistance genes from Solanum demissum were introduced in Solanum tuberosum. In this study we tried to isolate the gene fragments involved in potato resistance to Phytophthora infestans. We used potato plants (Solanum tuberosum) containing different resistance genes to Phytophthora infestans. After RAPD analyses of resistant and sensible potato, we have identified markers that were used as probe for Southern Blotting hybridization of digested and cloned genomic DNA of resistant potato plants. From bacterial colonies, identified as carrying the gene fragment involved in Phytophthora infestans resistance, was isolated plasmidial DNA. After selection of bacterial colonies, which presented, after amplification with specific primers of the vector, a singular band, the DNA was sequenced.
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