Genetic Structure at PRN-P Locus and Framing Risk Classes for Scrapie Resistance in Merinos of Cluj Breed
Abstract
The present paper shares the results of genetic structure analysis of the PRN-P gene, carried out in an ovine nucleus of Merinos of Cluj breed. Biological samples (blood) were collected from 20 rams (Merinos of Cluj breed) from a herd in genetics conservation belonging to SDE Cluj-Napoca and were genotyped at the PRN-P locus by means of primer extension analysis, and the final products were analyzed on a Genetic Analyzer ABI Prism 3130 xl. The frequency of genes by haplotypes were identified, and the individuals were categorized in risk groups based on the frequency of the identified genotype, with a view to applying assisted selection at the molecular level.
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