Cryopreservation of Rose Cultivars: Comparison of Two Encapsulation Protocols
AbstractShoot tips excised from in vitro plants of Rosa x hybrida L., cvs. Ioana, Mariana and Vulcan were successfully cryopreserved using encapsulation-dehydration and encapsulation-vitrification methods. Shoot tips (2-3 mm in length) were excised, encapsulated in natrium alginate beads, precultured in sucrose (0.25, 0.5, 0.75 M) for 24 h and either desiccated in laminar air flow (up to 5 h) or dehydrated with a vitrification solution (up to 220 min) prior to direct immersion in liquid nitrogen. The highest shoot development (72% for cv. Vulcan, 69% for cv. Mariana and 66% for cv. Ioana) after storage in liquid nitrogen was obtained after using the encapsulation-vitrification protocol.
a) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
b) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
c) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).