Comparative study of the effect of different anticoagulants on blood cell morphology in common carp (C. carpio) and rainbow trout (O. mykiss)
Abstract. Hematological assessment is an important tool in evaluating the fish health status. This tool corroborated with biochemical, parasitological and bacterial examinations aids the researchers in monitoring fish welfare status. For relevant blood samples using the right harvesting methodology with the right anticoagulant is imperative. This study presents a comparison between two different anticoagulants like Li-Heparin and ethylenediaminetetraacetic acid (EDTA) in common carp (Cyprinus carpio) and rainbow trout (Oncorhynchus mykiss). Two common carps and two rainbow trouts were submitted for blood harvesting using Li-Heparin and EDTA containers. Blood smears were prepared using a Romanowsky staining procedure and examined via light microscopy.
The results of the study revealed significant in vitro hemolysis and red blood cell morphologic alterations in the common carp blood samples when harvested on EDTA in contrast with the Li-Heparin anticoagulant. The white blood cells showed no significant changes induced by neither of the two anticoagulants. No significant anticoagulant induced cell morphological alterations were noticed in the rainbow trout. It is concluded that EDTA is not the appropriate anticoagulant for hematologic evaluation in common carp.
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